Bicarbonate And Fatty Acid Sythesis

Bicarbonate And Fatty Acid Sythesis-55
As shown in Table 1, addition of sodium bicarbonate to the nitrate and phosphate deficit cultures (NThe aim of the study was to investigate the effect of sodium bicarbonate on carbohydrate and lipid content of D. Among all macronutrient stresses, nitrogen stress amended with bicarbonate was found to be the ideal method for production of algal lipids, as it resulted in comparatively lower biomass with high carbohydrate and lipid contents in D. Effect of bicarbonate on protein, carbohydrate and lipid content of dry cell weight (DCW) of D. P cultures with or without bicarbonate did not have any significant effect on the protein content of D.salina compared to the standard control with adequate nutrients.salina during macronutrient deficit conditions (N). Under nutrient deficit conditions, addition of sodium bicarbonate (100 m M) significantly increased the biomass, carotenoids including β-carotene and lutein, lipid, and fatty acid content with concurrent enhancement of the activities of nutrient assimilatory and carbonic anhydrase enzymes.

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Sodium bicarbonate has also been found to increases their biochemical composition in nutrient deplete cultures thus making sodium bicarbonate, a convenient to transport and cost effective component providing a feasible alternative to gaseous inorganic carbon.

Only a limited number of microalgae have been investigated on the effect of sodium bicarbonate addition along with nitrate and phosphate deficient cultures and no report exists studying the effect of sodium bicarbonate under sulphate deficit growth conditions) which cause damage to cellular environment.

Figure S1 (Supplementary Material), depicts the chromatograph of individual carotenoid standards (lutein and β-carotene), Dunaliella cells were grown under total nutrient and nutrient deficit medium with or without sodium bicarbonate (Figs S2 and S3 of Supplementary Material).

The effect of bicarbonate on β-carotene and lutein of D.

Cytosolic acetyl-Co A carboxylase 1 (ACACA) catalyzes the reaction of bicarbonate, ATP, and acetyl-Co A to form malonyl-Co A, ADP, and orthophosphate. The human ACACA c DNA has been cloned (Abu-Elheiga et al. Four ACACA isoforms generated by alternative splicing have been identified as m RNAs - the protein product of the first has been characterized experimentally.

1995) and the biochemical properties of the human enzyme have recently been described (Cheng et al. ACACA uses biotin (Btn) and two Mn2 ions per subunit as cofactors and its activity is increased by polymerisation (Kim et al. Cytosolic ACACA is thought to maintain regulation of fatty acid synthesis in all tissues but especially lipogenic tissues such as adipose tissue and lactating mammary glands.

salina upon macronutrient stressed cultures was analyzed by HPLC (Fig. β-carotene and lutein content significantly increased in nutrient stressed cultures amended with bicarbonate compared to stressed cultures without bicarbonate. Values with different letters represent significantly differ at p As shown in Fig.

Highest accumulation of β-carotene was found in NEffect of bicarbonate on β-carotene and lutein of D. 2, the total lipid content was found to be high in both nutrient deficit cultures with and without bicarbonate compared to total nutrient.

Carbon dioxide can be taken up and utilized by microalgae in two forms, namely inorganic carbon (sodium bicarbonate) and carbon-dioxide gas.

Only a few algae have the ability to directly take up gaseous CO.

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