Persistence of Synaptic Facilitation for Learned Fear in : (1) it marks the activated synapse that confers synapse specificity, and (2) it stabilizes the synaptic growth associated with long-term facilitation.
We have found that a neuron-specific isoform of cytoplasmic polyadenylation element–binding protein (CPEB) regulates this synaptic protein synthesis in an activity-dependent manner.
The propensity of CPEB3 to form aggregates derive from its N-terminus domain which comprises two regions rich in glutamine and a low complexity sequence which is predicted to be poorly structured and to form aggregates.
To determine the role of CPEB3 in the persistence of synaptic plasticity and memory we generated a conditional knockout strain of CPEB3.
Dalí rendered his fantastic visions with meticulous verisimilitude, giving the representations of dreams a tangible and credible appearance.
In what he called "hand painted dream photographs," hard objects become inexplicably limp, time bends, and metal attracts ants like rotting flesh.
We found that CPEB3-mediated protein synthesis is required for maintenance, but not for memory acquisition.
We also found that CPEB3 loses its ability to maintain long-term synaptic plasticity and long-term memory if its prion-like N-terminus domain is deleted.
Similar to Aplysia, mouse CPEB3 forms aggregates upon synaptic activation in culture as well as performing a behavioral task in vivo.
Moreover the dual role in translation, the switch from repression to activation, is correlated with change of CPEB3 from a soluble to an aggregated form.