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You should also highlight any assumptions that you make about conditions during the research.You should set out your basic principles before embarking upon the experiment: any research will be built around some assumptions.
For a longer research paper, where you use an outline, it can be useful to structure your introduction around the outline. The introduction gives an overall review of the paper, but does address a few slightly different issues from the abstract.
The opening paragraphs of your paper will provide your readers with their initial impressions about the logic of your argument, your writing style, the overall quality of your research, and, ultimately, the validity of your findings and conclusions.
A vague, disorganized, or error-filled introduction will create a negative impression, whereas, a concise, engaging, and well-written introduction will lead your readers to think highly of your analytical skills, your writing style, and your research approach.
The introduction is the place to highlight any weaknesses in the experiment from the start.
For example, an ideal experiment should have perfectly randomized samples, but there are many good reasons why this is not always possible.